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Click Chemistry
The term 'click chemistry' describes a class of efficient and selective reactions capable to combine building blocks in a rapid fashion and at high yields.
| Excitation Light Sources | Characteristics | ||||||||||||||||||||
| Product Number (Specs Sheet) |
Product Name (Product Info) |
Target Group | 380 | 405 | 436 | 488 | 532 | 594 | 635 | 650 | 680 | 700 | 750 | 780 | Medium | λ abs [nm] |
ε [M –1 cm–1] |
λ em [nm] |
QY [%] |
FLT [ns] |
Buy |
| K7-567 | SeTau-405-Azide | triple-CC | • | • | PB 7.4 | 405 | 13,800 | 518 | 80 | 9.3 | |||||||||||
| K8-1346 | Seta-670-Azide | triple-CC | • | • | • | • | PB 7.4 | 667 | 180,000 | 686 | 7 | ||||||||||
| K8-1696 | Seta-633-Azide | triple-CC | • | • | PB 7.4 | 633 | 250,000 | 644 | 7 | ||||||||||||
| K8-3345 | Seta-555-DBCO | N3 | • | PB 7.4 | 555 | 155,000 | 570 | 7 | |||||||||||||
| K8-3346 | Seta-555-Azide | triple-CC | • | PB 7.4 | 555 | 155,000 | 570 | 7 | |||||||||||||
| K8-5045 | Seta-650-DBCO | N3 | • | • | PB 7.4 | 653 | 200,000 | 674 | 28 | ||||||||||||
| K8-5046 | Seta-650-Azide | triple-CC | • | • | PB 7.4 | 651 | 200,000 | 671 | 28 | ||||||||||||
| K8-7045 | Seta-750-DBCO | N3 | • | • | • | PB 7.4 | 754 | 234,000 | 782 | 21 | |||||||||||
| K8-7046 | Seta-750-Azide | triple-CC | • | • | • | PB 7.4 | 750 | 200,000 | 779 | 22 | |||||||||||
| K9-4154 new | SeTau-647-DBCO | N3 | • | • | PB 7.4 | 649 | 200,000 | 690 | 60 | 3.2 | |||||||||||
Azides:
Several of our specialty dyes are now available as azides to be reacted with C≡C-triple bonds in either a Cu(I)-catalized or Cu-free 1,3-dipolar cycloaddition reaction to a triazole (see figure). Both reactions proceed in aqueous environments with adequate efficiency.
Cu-catalyzed cycloaddition:
SeTau-405-azide is a clickable dye with a longer lifetime that is well suited for excitation with the blue, 404 nm diode laser. It has a large Stokes’ shift of over 110 nm, and the 8 ns lifetime make it also a valuable label for studying protein hydrodynamics (fluorescence polarization measurements of higher MW analytes). It is a good alternative for Pacific Orange to be used with Seta-405 in flow cytometry applications. Compatible with Fluorescein emission filter. Exc./Em. max: 405/518 nm.
The lifetime-sensitive Seta labels Seta-633-azide or Seta-670-azide exhibit shorter fluorescence lifetimes (FLTs) in aqueous media or when labeled to low molecular weight analytes (drugs or small ligands) but increased brightness and up to ten-times longer FLTs when bound to proteins or oligonucleotides and are unique tracers for FLT-based assays.
Seta-670-azide is also a label with extremly high 2PACS of 2000 GM at 840 nm for use in 2-photon microscopy.
Cyclo-Octyne (DBCO) Derivatives:
Strain-mediated cyclization reactions of cyclo-octynes proceed without the need for a Cu-catalyst. The metal-free reaction has wider applicability and is compatible with cell-based applications.
Cu-free, strain-mediated cycloaddition:
