tech1
$0.00

Superresolution Microscopy

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                   
K8-1357
K8-1357
Square-680-NHS
Square-680-NHS
NH2 MeOH 668 208000 690 10
Product Number
K8-1357
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K8-1384
K8-1384
Seta-700-NHS
Seta-700-NHS
NH2 PB 7.4 687 177000 703 6
Product Number
K8-1384
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K8-3335
K8-3335
Seta-555-NHS
Seta-555-NHS
NH2 PB 7.4 555 155000 570 7
Product Number
K8-3335
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K8-3345
K8-3345
Seta-555-DBCO
Seta-555-DBCO
N3 PB 7.4 555 155000 570 7
Product Number
K8-3345
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K8-3346
K8-3346
Seta-555-Azide
Seta-555-Azide
triple-CC PB 7.4 555 155000 570 7
Product Number
K8-3346
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K8-5035
K8-5035
Seta-650-NHS
Seta-650-NHS
NH2 PB 7.4 651 200000 671 28
Product Number
K8-5035
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K8-5036
K8-5036
Seta-650-Maleimide
Seta-650-Maleimide
SH PB 7.4 652 200000 672 28
Product Number
K8-5036
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K8-5045
K8-5045
Seta-650-DBCO
Seta-650-DBCO
N3 PB 7.4 653 200000 674 28 1
Product Number
K8-5045
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K8-5046
K8-5046
Seta-650-Azide
Seta-650-Azide
triple-CC PB 7.4 651 200000 671 28 1
Product Number
K8-5046
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K9-4119
K9-4119 new
SeTau-665-NHS
SeTau-665-NHS
NH2 PB 7.4 664 160000 712 53 3.1
Product Number
K9-4119
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K9-4142
K9-4142
SeTau-647-di-NHS
SeTau-647-di-NHS
NH2 PB 7.4 650 200000 694 65 3.2
Product Number
K9-4142
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K9-4145
K9-4145
SeTau-633-Ethyl-Ester
SeTau-633-Ethyl-Ester
CHCl3 634 88000 683 68
Product Number
K9-4145
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K9-4148
K9-4148
SeTau-647-Maleimide
SeTau-647-Maleimide
SH PB 7.4 648 200000 692 63 3.2
Product Number
K9-4148
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K9-4149
K9-4149
SeTau-647-NHS
SeTau-647-NHS
NH2 PB 7.4 649 200000 695 61 3.2
Product Number
K9-4149
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel
K8-1388
K8-1388
Seta-700-di-NHS
Seta-700-di-NHS
NH2 PB 7.4 688 180000 704 11
Product Number
K8-1388
Pack Size
Price
$0.00
Quantity
Total Price
$
Cancel

Novel Techniques:

Conventional microscopy is generally limited by the diffraction limit. In recent years a number of novel approaches have been developed to overcome the diffraction limit:

Near-field scanning optical microscopy (NSOM), stimulated emission depletion microscopy (STED), stochastic optical reconstruction microscopy (STORM) and structured illumination microscopy (SIM) are just a few of these new  techniques with improved lateral resolution down to tens of nanometers, more than an order of magnitude beneath that imposed by the diffraction limit, but each method has its limitations.

 

Squaraine-Rotaxane STED Dye:

SeTau-647-NHS was used for STED measurements of a HeLa-M cell nucleus.  Squaraine-rotaxanes are a new class of extremely bright and photostable dyes for fluorescence imaging and they are also well suited for STED. The images below show HeLa-M cell nuclei labeled with primary antibody mAB414. This antibody is well characterized, and labels the FG-repeat nucleoporins of mature nuclear pore complexes. The overall size of the individual NPC puncta on the nucleus is 100–120 nm. The excitation laser was 635 nm, and the STED depletion wavelength for this dyes is 770 nm. The emission filter was 685/40.

SeTau-647-Conventional-Image-249x249.gif

Conventional Image

SeTau-647-STED-Image-249x249.gif

STED Image

(Courtesy of J. Bewersdorf & J. Meyers, Yale University)

 

Please provide following information to download software:

Your Name*:
Email ID*:
Contact Number:
Cancel