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SeTau Lifetime Labels

 

Fluorescent labels with long lifetimes

Excitation Light Sources Characteristics Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Medium λ abs
[nm]
ε
[M–1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
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K1-204
K1-204
SeTau-380-NHS
SeTau-380-NHS
NH2 Water 270 23800 480 56 32.5 BSA, D/P=1 383 23800 468 44 30.6
Product Number
K1-204
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K7-547
K7-547
SeTau-405-NHS
SeTau-405-NHS
NH2 PB 7.4 405 13800 518 80 9.3 IgG, D/P=8 405 13800 518 19 8.4
Product Number
K7-547
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K7-567
K7-567
SeTau-405-Azide
SeTau-405-Azide
triple-CC PB 7.4 405 13800 518 80 8 EtOH 391 15000 498 55 8.5
Product Number
K7-567
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K7-545
K7-545
SeTau-425-NHS
SeTau-425-NHS
NH2 PB 7.4 425 4200 545 39 26.2 BSA, D/P=1 427 4200 528 16 26.2
Product Number
K7-545
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K9-4119
K9-4119 new
SeTau-665-NHS
SeTau-665-NHS
NH2 PB 7.4 664 160000 712 53 3.1 IgG, D/P=1.5 662 160000 716 46 3.1
Product Number
K9-4119
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K9-4142
K9-4142
SeTau-647-di-NHS
SeTau-647-di-NHS
NH2 PB 7.4 650 200000 694 65 3.2 IgG, D/P=0.5 649 200000 691 58 3.2
Product Number
K9-4142
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K9-4148
K9-4148
SeTau-647-Maleimide
SeTau-647-Maleimide
SH PB 7.4 648 200000 692 63 3.2 PB 7.4 648 200000 692 63 3.2
Product Number
K9-4148
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K9-4149
K9-4149
SeTau-647-NHS
SeTau-647-NHS
NH2 PB 7.4 649 200000 695 61 3.2 IgG, D/P=1 648 200000 694 59 3.3
Product Number
K9-4149
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K7-548
K7-548
SeTau-405-Maleimide
SeTau-405-Maleimide
SH PB 7.4 405 13800 518 51 9.1 EtOH 391 15000 498 32 8.5
Product Number
K7-548
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K7-544
K7-544
SeTau-425-Maleimide
SeTau-425-Maleimide
SH PB 7.4 425 4200 545 39 26.2 PB 7.4 425 4200 545 39 26.2
Product Number
K7-544
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K9-4169
K9-4169 new
SeTau-670-NHS
SeTau-670-NHS
NH2 PB 7.4 673 275000 694 36 1.6 IgG D/P = 4 672 693 14 1.75
Product Number
K9-4169
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Short-Wavelength SeTau Series: FLT and Polarization labels

These labels with longer lifetimes in the range between 9 and 32 ns are excitable in the UV or blue region and are useful as polarization probes for the measurement of high MW analytes (small proteins) or for lifetime-based applications. They exhibit long lifetimes that are not environment sensitive.

SeTau-380-NHS is a reactive dye with a with a large Stokes’ shift of 210 nm and a fluorescence lifetime of 32.5 ns in water.  Exc./Em. max: 270/480 nm.

SeTau-405-NHS is a dye that is well suited for excitation with the blue 404 nm diode laser. Because of its large Stokes’ shift of more than 110 nm, the quenching tendency upon labeling to proteins is very low. Importantly the fluorescence lifetime of this dye is in the order of 9 ns, making it also a valuable label for studying protein hydrodynamics (fluorescence polarization measurements). It is a good alternative for Pacific Orange to be used with Seta-405 in flow cytometry applications. Compatible with Fluorescein emission filter. Exc./Em. max: 405/518 nm.

SeTau-425-NHS was used to measure high-molecular-weight analytes such as BSA directly in an FP assay (more under Fluorescence Polarization). Importantly the FLT of these labels does not change upon covalent attachment to proteins or other analytes. For more information we refer you to Lifetime Applications.
 

 SeTau FLT FD  SeTau FLT TD

 

Long-Wavelength SeTau Series: FLT and Polarization labels

The longer lifetimes (in the order of 2 – 4 ns, see below) and larger Stokes' shifts (40 − 50 nm) of squaraine rotaxanes compared to conventional cyanine based NIR labels (τ = 0.5 – 1.5 ns) make them in particular suitable as labels for use in fluorescence polarization immunoassays. The squaraine rotaxane dye SeTau-647 is currently the brightest label on the market for the Kr-ion (647 nm) laser line.

 

SeTau-647-IgG vs. DP

Quantum yields of IgG-conjugates of SeTau-647, Alexa 647 and Cy5 for different
dye-to-protein ratios in phosphate buffer (pH 7.4)

 

 squ1  "SeTau-647,Cy5,Alexa-647.png"
Chemical stability plot of SeTau-647 vs. Cy5
in presence of ozone or H2O2
Fluorescence Lifetime of SeTau-647 vs Alexa
647 or Cy5 in water

 

 

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