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pH Sensing Applications

Characteristics Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Medium λ abs
[nm]
ε
[M–1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
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K8-1367
K8-1367
Seta-670-pH-di-NHS
Seta-670-pH-di-NHS
NH2 PB 6.0 672 93000 694 19 IgG, D/P=1 684 703 7
Product Number
K8-1367
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K8-1407
K8-1407
Square-650-pH-NHS
Square-650-pH-NHS
NH2 PB 5.6 653 135000 671 16 1.17 IgG, D/P=0.8
pH=2.0
662 135000 677 7 1.52
Product Number
K8-1407
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K8-1667
K8-1667
Seta-635-pH-di-NHS
Seta-635-pH-di-NHS
NH2 PB 7.4 640 188000 656 33 1.8 IgG, D/P=1 646 663 21
Product Number
K8-1667
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K8-1405
K8-1405
Square-650-pH-Carboxy
Square-650-pH-Carboxy
NH2 PB 5.6 653 135000 671 16 1.17 pH 9.0 535 48000 663 9 0.53
Product Number
K8-1405
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K8-1610
K8-1610
Square-635-Carboxy
Square-635-Carboxy
pH 7.4 634 180000 646 9 pH 12.0 520 - - -
Product Number
K8-1610
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K8-1612
K8-1612
Square-635-NHS
Square-635-NHS
NH2 pH 7.4 634 180000 646 9 BSA, D/P=1.0 649 663 29
Product Number
K8-1612
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K8-1665
K8-1665
Seta-635-pH-di-Carboxy
Seta-635-pH-di-Carboxy
PB 7.4 640 188000 656 33 1.8 pH 12.0 519 - - -
Product Number
K8-1665
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K8-1365
K8-1365
Seta-670-pH-di-Carboxy
Seta-670-pH-di-Carboxy
PB 6.0 672 93000 694 19 pH 11.5 535 - - -
Product Number
K8-1365
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K8-1613
K8-1613
Square-635-Maleimide
Square-635-Maleimide
SH pH 7.4 636 180000 649 8
Product Number
K8-1613
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pH-measurement in cells: Probes such as Square-650-pH-Carboxy and labels such as Square-650-pH-NHS exhibit pKa's in the physiologically relevant pH range and are therefore useful to measure the intercellular pH changes in cells. For more information about these dyes we refer you to the specs sheet of Square-650-pH-Carboxy and Square-650-pH-NHS and the published literature.

 

Square-650-pH-EmSpectrum-vs-pH Square-650-pH: Em vs pH

pH-probes with alkaline pKa's: The pH-sensitive dyes with pKa's in the alkaline pH range (see table below) are available in probe and reactive formats. Their pKa's range from 8.8 to 10.7.

 Em-vs-pH

 

Product Number
(Specs Sheet)
Product Name
(Product Info)
AvailabilityProtonated formDeprotonated
form
pKapH range
λ abs
[nm]
λ em
[nm]
QY
[%]
λ abs (λ em)
[nm]
K8-1365 Seta-670-pH-di-Carboxy ~ 2 weeks 672 694 19 535

8.8

9.6

7.6–11.4
K8-1367 Seta-670-pH-di-NHS ~ 2 weeks
IgG conjugate, D/P=1 684 703 7
K8-1405 Square-650-pH-Carboxy ~ 2 weeks 653 671 16 535 (663) 7.1 5.3-9.0
K8-1407 Square-650-pH-NHS in stock
IgG conjugate, D/P=1 662 677 7 546 (663) 6.3 4.0–8.0
K8-1610 Square-635-Carboxy in stock 634 646 9 520 10.7 9.6–11.7
K8-1612 Square-635-NHS in stock
K8-1613 Square-635-Maleimide in stock 636 649 8
BSA conjugate, D/P=1

649 663 29
K8-1665 Seta-635-pH-di-Carboxy ~ 2 weeks 640 656 33 519 10.2 9.2-11.8
K8-1667 Seta-635-pH-di-NHS ~ 2 weeks
IgG conjugate, D/P=1 646 663 21

 

Color-vs-pH(516x155)

Comparison of the dyes Seta-646-NHS, Seta-670-pH-di-NHS and Seta-635-pH-di-NHS
in a range of universal buffered solutions (pH 5.28 – 12.69)

 

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