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NIR Fluorescence Imaging

 

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Fluorescence imaging utilizes light produced by fluorescent species that are introduced into the specimen of interest. The near-infrared (NIR) region offers advantages for photon penetration using exogenous contrast agents. Near-infrared optical imaging technologies are based on the light producing capabilities of extrinsic fluorescent molecules such as fluorescent proteins or fluorescent dyes. NIR imaging helps to overcome some of the shortcomings of imaging with conventional dyes which have limitations due to their shorter excitation wavelengths. It is known that the penetration of light through tissue is indirectly proportional to the fourth power of the wavelength used, and as such NIR light has the capability to penetrate tissue more efficiently than light in the visible range. Also there is less background fluorescence in the NIR region.

Seta Dye NIR fluorescent labeling reagents provide a flexible solution for labeling proteins, peptides or antibodies. Available as 680 nm, 700 nm, 750 nm and 780 excitable dyes, these Seta Dye labeling reagents are ideal for in vivo optical imaging and fully compatible with the KODAK In-Vivo Multispectral Imaging System FX, the IVIS Imaging System (Caliper LifeSciences) or the Odyssey Fc Imaging System (Li-COR).

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
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K8-1384
K8-1384
Seta-700-NHS
Seta-700-NHS
NH2 PB 7.4 687 177000 703 6
Product Number
K8-1384
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K8-5075
K8-5075
Seta-680-NHS
Seta-680-NHS
NH2 PB 7.4 678 200000 698 24
Product Number
K8-5075
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K8-7055
K8-7055
Seta-780-NHS
Seta-780-NHS
NH2 PB 7.4 777 215000 807 1.2
Product Number
K8-7055
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K9-4119
K9-4119 new
SeTau-665-NHS
SeTau-665-NHS
NH2 PB 7.4 664 160000 712 53 3.1
Product Number
K9-4119
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K8-1388
K8-1388
Seta-700-di-NHS
Seta-700-di-NHS
NH2 PB 7.4 688 180000 704 11
Product Number
K8-1388
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K8-7522
K8-7522
Seta-750-NHS
Seta-750-NHS
NH2 PB 7.4 753 230000 780 14
Product Number
K8-7522
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K8-1389
K8-1389 new
Seta-700-NHS
Seta-700-NHS
NH2 PB 7.4 688 162000 703 14
Product Number
K8-1389
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$0.00
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K9-4169
K9-4169 new
SeTau-670-NHS
SeTau-670-NHS
NH2 PB 7.4 673 275000 694 36 1.6
Product Number
K9-4169
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Seta-680-NHS is an amine-reactive NIR fluorescent dye with absorption and emission at 680 and 700 nm. The dye is fully compatible with the IVIS Imaging System (Caliper) or Odyssey Fc Imaging System (Li-Cor). Its reactive dye content is optimized to be at least 75%. Seta-680-NHS and our other near IR dyes are ideal for in vivo imaging due to increased penetration of tissue in this long wavelength range.

Seta-700-di-NHS is an amine-reactive NIR fluorescent dye with absorption and emission at 687/703 nm. It is efficiently excited at 680 nm and it shows high quantum yields up to 25% upon binding to antibodies or other proteins. Its reactive dye content is optimized to be at least >75%. Seta-700-di-NHS and our other near IR dyes are ideal for in vivo imaging.

Seta-750-NHS is an amine-reactive NIR fluorescent dye with absorption and emission at 750 and 780 nm. It is extremely bright and photostable. Its minimum reactive dye content is optimized to be at least 75%. Seta-750-NHS and our other NIR dyes are ideal for in vivo imaging due to increased penetration of excitation light through tissue in this long wavelength range. This dye also has been successfully used for in vivo analysis of the biodistribution of fluorescently labelled PEG-single wall carbon nanotubes (PEG-SWCNTs). (L. Campagnolo et al. Biodistribution and toxicity of pegylated single wall carbon nanotubes in pregnant mice. Particle and Fibre Toxicology 201310:21).

Seta-780-NHS is an amine-reactive, NIR fluorescent dye with absorption and emission at 777 and 807 nm. Its reactive dye content is optimized to be at least 75%. Seta-780-NHS and our other NIR dyes are ideal for in vivo imaging due to increased penetration of excitation light through tissue in this long wavelength range.

 

See also Fluorescence Imaging and Fluorescence Lifetime Imaging.

 

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