SETA BioMedicals

Flow Cytometry

Flow Cytometry

Flow Cytometry is a powerful technique to measure multiple parameters from cell populations. Flow cytometers differentiate cells based on their size, internal complexity, and fluorescence signature. Qualitative and quantitative analyses of cell populations have relevance for clinical and research applications.

SETA BioMedicals offers a variety of proprietary labels for flow cytometry applications, some of them with large Stokes’shifts of 50 to 60 nm. A table listing the replacements for the most common fluorophores currently used is given below. Our fluorescent labels are extremely bright and photostable. We refer you to our specification sheets for more detailed information and a direct comparison with commonly used labels including brightness and stability. We offer you guaranteed the most reliable and affordable fluorescent labels for your application. Please contact us at info@setabiomedicals.com or call us at (217) 417 2160 if you cannot find a replacement on the table below.

Laser Source	Labels Used	Proposed Superior Seta Replacements
405 nm	Pacific Blue	Seta-409
405 nm	Pacific Orange	SeTau-405
488 nm	PE-Cy5	PE-Seta 633 or PE-Seta-650 or PE-Seta-646
	PE-Alexa-700	PE - SeTau-665 with emission at 710 nm
	PerCP - Cy5.5	PerCP - SeTau-665 or PerCP-Seta-680
	PE - Cy5.5	PE - Seta-680 and PE - SeTau-665
	PE-Cy7	PE - Seta-750
633 nm	APC	Seta-646
	Alexa 647	Seta-633, Seta-650 , Seta-660, SeTau-665
	Cy5	Seta-633, Seta-650 , Seta-660, SeTau-665
	Alexa 700	SeTau-665 , SeTau-647
	APC - Cy5.5	APC - SeTau-665 , APC - Seta-680, APC - SeTau-647
	APC - Cy7	APC - Seta-750

SETA BioMedicals also provides tandem-conjugates of these dyes with phycobiliproteins. Please contact us for more information.

Product Information:

Product	Pack Size	Product Code
Seta-750-mono-NHS	1 mg	K8-7035-1mg
SeTau-665-mono-NHS	0.5 mg	K9-4119-0.5mg
Seta-650-mono-NHS	1 mg	K8-5035-1mg
Seta-660-mono-NHS	1 mg	K8-1682-1mg
SeTau-647-di-NHS	0.5 mg	K9-4142-0.5mg
SeTau-647-mono-NHS	0.5 mg	K9-4149-0.5mg
Seta-646-mono-NHS	1 mg	K8-1672-1mg
Seta-633-mono-NHS	1 mg	K8-1663-1mg
Seta-632-mono-NHS	1 mg	K8-1642-1mg
Seta-404-mono-NHS	1 mg	K7-547-1mg

Pricing and Availability
Ordering

Product Specifications:


			Excitation Light Sources								Free Dye					Dye Conjugate
Product Number	Product Name	Target Group	380	405	436	488	532	594	635	670	Solvent	λ_Ab max. [nm]	ε [M^–1cm^–1]	λ_Em max. [nm]	QY [%]	Carrier PB 7.4	λ_Ab max. [nm]	λ_Em max. [nm]	QY [%]
K7-547	SeTau 405 NHS	NH₂	•	•							PB 7.4	405	13800	518	80	BSA, D/P=8	406	518	20
K8-1342	Seta-670-NHS	NH₂		•					•	•	PB 7.4	667	179000	688	7	BSA, D/P=1	681	695	35
K8-1351	Square-660-COOH		•	•					•	•	PB 7.4	658	182000	678	3	6g/L BSA	679	696	24
K8-1352	Square-660-NHS	NH₂		•						•	PB 7.4	658	182000	677	3	BSA, D/P=1	676	695	13
K8-1355	Square-670H-COOH		•	•						•	MeOH	668	208000	690	10	1g/L BSA	688	708	62
K8-1500	Square-655		•	•						•	MeOH	628	177000	654	0.09	1 g/L BSA	652	672	80
K8-1641	Seta-632-Maleimide	SH							•		PB 7.4	633	270000	642	5
K8-1642	Seta-632-NHS	NH₂							•		PB 7.4	632	280000	641	6	IgG, D/P=1	637	647	18
K8-1663	Seta-633-NHS	NH₂							•		PB 7.4	633	250000	644	7	IgG, D/P=1	637	647	26
K8-1672	Seta-646-NHS	NH₂							•		PB 7.4	646	207000	656	10	IgG, D/P=1	650	661	33
K8-1682	Seta-660-di-NHS	NH₂							•		PB 7.4	661	151000	672	11	IgG, D/P=1	665	675	49
K8-3335	Seta-555-NHS	NH₂					•				PB 7.4	556	155000	570	12	IgG, D/P=1	654	571	23
K9-4112	SeTau-655-di-NHS	NH₂							•		PB 7.4	665	200000	673	25	IgG, D/P=1	657	675	31
K9-4113	SeTau-665-di-NHS	NH₂	•							•	PB 7.4	665	140000	715	62	IgG, D/P=1	664	709	43
K9-4142	SeTau-647-di-NHS	NH₂							•		PB 7.4	650	368000	694	65	IgG, D/P=0.5	649	691	58
K8-5035	Seta-650-NHS	NH₂							•		PB 7.4	651	172000	671	28	IgG, D/P=1	654	673	37
K8-7035	Seta-750-NHS	NH₂								•	PB 7.4	750	235000	779	22	IgG, D/P=1	755	783	24
K8-1350	Square-670-Carboxy		•						•	•	PB 7.4	662	207000	680	1.7	6 g/L BSA	658	676	29
K8-1300	Square-670-di-COOH		•	•						•	PB 7.4	667	207000	685	7	6g/L BSA	678	698	28
K8-5036	Seta-650-maleimide	SH							•		PB 7.4	652	210000	672	28
K8-7038	Seta-750-Maleimide	SH								•	PB 7.4	752	235000	779	22	IgG, D/P=1	755	783	24
K9-4149	Seta-647-NHS	NH2							•		PB 7.4	649	200000	695	61	IgG, D/P=1	648	694	59
K9-4119	SeTau-665-NHS	NH2	•	•						•	PB 7.4	664	160000	712	53	IgG, D/P=1.2	653	716	50
K8-1407	Square-650-pH-NHS								•		PB 7.4	653	135000	671	16	IgG, D/P=1	662	677	7