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Long-wavelength SeTau Series |
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Long-wavelength SeTau Series: Squaraine-Rotaxanes
The long-wavelength SeTau dye series is a new, next generation class of dyes based on Squaraine-rotaxanes that combines extremely high quantum yields in water (up to 60%) and high extinction coefficients in the order of 150 - 365,000 M-1. cm-1. These dyes were developed by SETA BioMedicals in order to overcome some of the shortcomings of conventional dyes such as low sensitivity, low stability against oxygen species and low photostability. SeTau dyes are available in reactive forms with carboxy, NHS ester and maleimide functionalities but also as hydrophobic probes for imaging.
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| The longer lifetimes of squaraine rotaxane in the order of 2 - 4 ns compared to conventional cyanine based NIR labels (τ = 0.5 – 1.5 ns) make them in particular suitable as labels for use in fluorescence polarization immunoassays. Please visit our website or call us at 217 417 2160 for more information. |
| Sample | Absorption
max.
[nm] | Extinction
Coefficient
[M–1.cm–1] | Emission
max.
[nm] | Quantum
Yield
[%] |
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650
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368,000
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694
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65
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665
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150,000
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716
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60
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The next-generation squaraine rotaxane dye SeTau 647 (K9-4149) is currently the brightest label on the market for the Kr-ion (647 nm) laser line.
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Quantum yields of IgG-conjugates of SeTau 647, Alexa 647 and Cy5 at various
dye-to-protein ratios in phosphat buffer (pH 7.4)
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Chemical stability plot of SeTau 665 vs. Cy5
in presence of ozone or H2O2.
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Fluorescence Lifetime of SeTau 665 vs Alexa
647 or Cy5 in water |
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Hydrophilic squaraine-rotaxane labels:
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| Product | Lifetime [ns] | Pack Size | Product Code |
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3.2
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0.5 mg
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K9-4149-0.5mg
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3.2
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0.5 mg
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K9-4142-0.5mg
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3.1
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0.5 mg
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K9-4119-0.5mg
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3.1
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0.5 mg
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K9-4113-0.5mg
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Pricing and Availability
Ordering
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| Product Specifications: |
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Excitation Light Sources |
Free Dye |
Dye Conjugate |
| Product Number |
Product Name |
380 | 405 |
436 | 488 | 532 | 594 | 635 | 670 | Solvent | λAb max. [nm] | ε [M–1
cm–1] |
λEm max. [nm] | QY [%] |
Carrier
PB 7.4 | λAb max. [nm] | λEm max. [nm] | QY [%] |
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| K9-4112 |
SeTau-655-di-NHS |
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PB 7.4 |
665 |
200000 |
673 |
25 |
IgG, D/P=1 |
657 |
675 |
31 |
| K9-4113 |
SeTau-665-di-NHS |
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PB 7.4 |
665 |
140000 |
715 |
62 |
IgG, D/P=1 |
664 |
709 |
43 |
| K9-4142 |
SeTau-647-di-NHS |
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PB 7.4 |
650 |
368000 |
694 |
65 |
IgG, D/P=0.5 |
649 |
691 |
58 |
| K9-4149 |
Seta-647-NHS |
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PB 7.4 |
649 |
200000 |
695 |
61 |
IgG, D/P=1 |
648 |
694 |
59 |
| K9-4119 |
SeTau-665-NHS |
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PB 7.4 |
664 |
160000 |
712 |
53 |
IgG, D/P=1.2 |
653 |
716 |
50 |
| K8-1384 |
Square-690-mono-NHS |
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PB7.4 |
687 |
130000 |
703 |
13 |
IgG, D/P = 1 |
704 |
716 |
72 |
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