SETA BioMedicals

Fluorescence Imaging

Fluorescence Imaging

Fluorescence imaging utilizes light produced by fluorescent species that are introduced into the specimen of interest. The near-infrared (NIR) region offers advantages for photon penetration using exogenous contrast agents. Near-infrared optical imaging technologies are based on the light producing capabilities of extrinsic fluorescent molecules such as fluorescent proteins or fluorescent dyes. NIR imaging helps to overcome some of the shortcomings of conventional dyes which have limitations due to their shorter excitation wavelengths. It is known that the penetration of light through tissue is indirectly proportional to the fourth power of the wavelength used, and as such NIR light has the capability to penetrate tissue more efficiently than light in the visible range. In addition there is less background fluorescence in the NIR region.

Seta Fluor NIR fluorescent labeling reagents provide a flexible solution for labeling proteins, peptides or antibodies. Available as 665 nm, 680 nm, 750 nm or 780 nm excitable dyes, Seta Fluor kits are ideal for in vivo optical imaging and compatible with the following instruments: IVIS Imaging Systems (Caliper LifeSciences) or the Odyssey Fc Imaging System (Li-COR).

SeTau-665-NHS is an amine-reactive NIR fluorescent dye with absorption and emission at 665/708 nm. It is efficiently excited at 680 nm and its large Stokes shift allows to utilize broader emission filters and to increase the sensitivity of your measurements. Its reactive dye content is optimized to be at least >75%. It is twice a bright as conventional fluorescent dyes in this wavelength range (Q.Y. ~ 60%). Seta 665 NHS and our other near IR dyes are ideal for in vivo imaging.

Seta 680 NHS ester is an amine-reactive NIR fluorescent dye with absorption and emission at 680 and 700 nm. The dye is fully compatible with the IVIS (Caliper) or Odyssey Imaging system (Li-Cor). Its reactive dye content is optimized to be at lest 75%. Seta 680 NHS and our other near IR dyes are ideal for in vivo imaging due to increased penetration of tissue in this long wavelength range.

Seta-750-mono-NHS is an amine-reactive NIR fluorescent dye with absorption and emission at 650 and 780 nm. It is extremely bright and photostable. Its minimum reactive dye content is optimized to be at least 75%. Seta-750-mono-NHS and our other near IR dyes are ideal for in vivo imaging due to increased penetration of excitation light through tissue in this long wavelength range. The dye is fully compatible with the IVIS (Caliper) or Odyssey Imaging system (Li-Cor).

Seta 780 NHS ester is an amine-reactive, NIR fluorescent dye with absorption and emission at 680 and 800 nm. Its reactive dye content is optimized to be at least 75%. Seta 780 NHS and our other near IR dyes are ideal for in vivo imaging due to increased penetration of excitation light through tissue in this long wavelength range. The dye is fully compatible with the IVIS (Caliper) or Odyssey Imaging system (Li-Cor).

Product Information:

Product	Pack Size	Product Code
SeTau-665 NHS	0.5 mg	K9-4119-0.5mg
Seta-680 NHS	0.5 mg	K8-5085-0.5mg
Seta-750 NHS	0.5 mg	K8-7035-0.5mg
Seta-780 NHS	0.5 mg	K8-7085-0.5mg

Pricing and Availability
Ordering

Product Specifications:


		Excitation Light Sources								Free Dye					Dye Conjugate
Product Number	Product Name	380	405	436	488	532	594	635	670	Solvent	λ_Ab max. [nm]	ε [M^–1cm^–1]	λ_Em max. [nm]	QY [%]	Carrier PB 7.4	λ_Ab max. [nm]	λ_Em max. [nm]	QY [%]
K8-7035	Seta-750-NHS								•	PB 7.4	750	235000	779	22	IgG, D/P=1	755	783	24
K9-4119	SeTau-665-NHS	•	•						•	PB 7.4	664	160000	712	53	IgG, D/P=1.2	653	716	50
K8-xxxx	Seta xxx										388	159000	544	65