Increasing the sensitivity of measurement of the insulin secretion dynamics with SeTau-647
Due to SeTau-647 having a longer lifetime and higher brightness compared to the previously used Cy5 fluorophore, a 45% increase in the anisotropy range was observed with enhanced signal-to-noise ratio (S/N) of the measurements. This new system was tested by measuring glucose-stimulated insulin secretion from single and groups of murine and human islets. Mathematical derivations for immunoassay equilibrium equations are provided as well as a table containing the properties of SeTau-647 and Cy5. Adablah et al., Anal. Chem. 2020, 92, 12, 8464–8471.
| Excitation Light Sources | Characteristics | ||||||||||||||||||||
| Product Number (Specs Sheet) |
Product Name (Product Info) |
Target Group | 380 | 405 | 436 | 488 | 532 | 594 | 635 | 650 | 680 | 700 | 750 | 780 | Medium | λ abs [nm] |
ε [M –1 cm–1] |
λ em [nm] |
QY [%] |
FLT [ns] |
Buy |
| K9-4119 new | SeTau-665-NHS | NH2 | • | • | • | PB 7.4 | 664 | 160000 | 712 | 53 | 3.1 | ||||||||||
| K9-4148 | SeTau-647-Maleimide | SH | • | • | PB 7.4 | 648 | 200000 | 692 | 45 | 3.2 | |||||||||||
| K9-4149 | SeTau-647-NHS | NH2 | • | • | PB 7.4 | 649 | 200000 | 695 | 61 | 3.2 | |||||||||||
| K9-4169 new | SeTau-670-NHS | NH2 | • | • | • | PB 7.4 | 673 | 275000 | 694 | 36 | 1.6 | ||||||||||
| K9-4179 new | SeTau-680-NHS | NH2 | • | • | • | PB 7.4 | 683 | 295000 | 705 | 58 | 2.9 | ||||||||||
