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  • Longest single-fluorescent-molecule tracking ever reported with SeTau-647

Longest single-fluorescent-molecule tracking ever reported with SeTau-647

The photobleaching rates of 13 fluorescent dyes, 4 of them conjugated to trolox, were investigated for single-molecule measurements at 37oC in living cells. Among these SeTau-647 (K9-4149 and K9-4148), a squaraine rotaxane dye, exhibited the best photobleaching performance in this study with an exponential lifetime of 105.8 sec. As a membrane-impermeable dye it allowed to observe up to 12,000 frames, which is the longest single-fluorescent-molecule tracking ever reported. See paper in Nat. Chem. Biol. 14, 497–506 (2018) [32].

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                   
K9-4119
K9-4119 new
SeTau-665-NHS
SeTau-665-NHS
NH2 PB 7.4 664 160000 712 53 3.1
K9-4148
K9-4148
SeTau-647-Maleimide
SeTau-647-Maleimide
SH PB 7.4 648 200000 692 45 3.2
K9-4149
K9-4149
SeTau-647-NHS
SeTau-647-NHS
NH2 PB 7.4 649 200000 695 61 3.2
K9-4169
K9-4169 new
SeTau-670-NHS
SeTau-670-NHS
NH2 PB 7.4 673 275000 694 36 1.6
K9-4179
K9-4179 new
SeTau-680-NHS
SeTau-680-NHS
NH2 PB 7.4 683 215000 705 58 2.9

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