Product Name : Seta-633-di-NHS

Product Number:

K8-1626

Target Group:

NH₂

Excitation Light Sources:

Medium 1:

PB 7.4

λ_Ab max. [nm]:

635

ε [M^–1cm^–1]:

192000

λ_Em max. [nm]:

644

QY [%]:

FLT [ns]:

0.7

Medium 2:

IgG, D/P=1

λ_Ab max. [nm]:

639

ε [M^–1cm^–1]:

192000

λ_Em max. [nm]:

650

QY [%]:

FLT [ns]:

Specs Sheet:

Description

Highly hydrophilic, amine-reactive label containing two reactive NHS-ester groups.

Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides.
Fluorescence intensity and fluorescence polarization-based applications.
Resonance Energy Transfer (RET).
Flow Cytometry.
Immunofluorescence.
Homogeneous Assays.

Perfectly suited for excitation with the 635-nm diode laser.
Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules.
Low non-specific binding.
pH-insensitive between pH 3 and pH 10.
Good aqueous solubility; this label does not alter the solubility of the bioconjugate.
High photostability compared to fluorescein, Cy5 or Alexa Fluor 647.
Low molecular weight — Seta dyes do not add substantial mass to the conjugates.
Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids.