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Product Name : Seta-633-di-NHS

Product Number:
K8-1626
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
635
ε [M–1 cm–1]:
192000
λEm max. [nm]:
644
QY [%]:
12
FLT [ns]:
0.7
Medium 2:
IgG, D/P=1
λAb max. [nm]:
639
ε [M–1 cm–1]:
192000
λEm max. [nm]:
650
QY [%]:
33
FLT [ns]:
3
Specs Sheet:
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Description

  • Highly hydrophilic, amine-reactive label containing two reactive NHS-ester groups.

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides.
  • Fluorescence intensity and fluorescence polarization-based applications.
  • Resonance Energy Transfer (RET).
  • Flow Cytometry.
  • Immunofluorescence.
  • Homogeneous Assays.

 

Advantages

  • Perfectly suited for excitation with the 635-nm diode laser.
  • Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules.
  • Low non-specific binding.
  • pH-insensitive between pH 3 and pH 10. 
  • Good aqueous solubility; this label does not alter the solubility of the bioconjugate. 
  • High photostability compared to fluorescein,  Cy5 or Alexa Fluor 647.
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates. 
  • Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids.