A highly sensitive and selective probe for Vancomycin based on Seta-640 (K8-1662)
In the absence of Vancomycin, the close proximity and efficient electron-transfer from Seta-640 (K8-1662) to anthraquinone causes significant fluorescence quenching of the dye (“off”-state). When Vancomycin is added, it binds with high affinity in a 2:1 ratio to the -d-Ala-d-Ala ligands, resulting in a 30 times increase in fluorescence intensity (“on”-state).
Excitation Light Sources
Characteristics
Product Number
(Specs Sheet)Product Name
(Product Info)Target Group
380
405
436
488
532
594
635
650
680
700
750
780
Medium
λ abs
[nm]ε
[M –1
cm–1]λ em
[nm]QY
[%]FLT
[ns]Buy
K8-1662
Seta-640-di-NHS
NH2
•
PB 7.4
636
181000
646
13
0.7
Citation: Vancomycin determination by disrupting electron-transfer in a fluorescence recovery turn-on Squaraine -Anthraquinone Triad. Shue Mei Ng et al. ACS Sens. 2018, 3, 6, 1156.
