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  • A highly sensitive and selective probe for Vancomycin based on Seta-640 (K8-1662)

A highly sensitive and selective probe for Vancomycin based on Seta-640 (K8-1662)

In the absence of Vancomycin, the close proximity and efficient electron-transfer from Seta-640 (K8-1662) to anthraquinone causes significant fluorescence quenching of the dye (“off”-state). When Vancomycin is added, it binds with high affinity in a 2:1 ratio to the -d-Ala-d-Ala ligands, resulting in a 30 times increase in fluorescence intensity (“on”-state).

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                   
K8-1662
K8-1662
Seta-640-di-NHS
Seta-640-di-NHS
NH2 PB 7.4 636 181000 646 13 0.7

Citation: Vancomycin determination by disrupting electron-transfer in a fluorescence recovery turn-on Squaraine -Anthraquinone Triad. Shue Mei Ng et al. ACS Sens. 2018, 3, 6, 1156.

 

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