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Overview

SETA BioMedicals offers and commercializes fluorescent products for a broad range of applications in the biomedical, pharmaceutical, and environmental research fields.

These products can be categorized according to their functional use into dyes, probes and labels, fluorescent proteins and conjugates.

Fluorescent Labels:

Square, Seta, and SeTau dyes are a series of dyes that span the whole visible spectrum and the NIR. They are available with various functionalities: carboxylic acids, as amine-reactive NHS esters for labeling of amino groups on peptides, proteins or amino-modified DNA, as maleimides for coupling to thiol-groups in proteins and thiol-modified oligos and as-amino-modified labels (on request).

With a few exceptions all these reagents are available in either 0.5 mg, 1 mg or 5 mg Pack Sizes. Please do inquire about the availability of bulk quantities.



Labeling of Amino-groups:


The following functional groups available for labeling of amino-groups in proteins or amino-modified oligos:

overview

Among all these functionalities N-hydroxysuccinimide (NHS) ester are the preferred reactive functionality for labeling of amino-groups due to the formation of highly stable amide bonds with amines. Sulfonyl chlorides also form stable sulfamide bonds but the reaction also generates HCl, which is a not so desirable by-product. In addition sulfonoyl chlorides are more difficult to synthesize.

Labelling of Thiol-groups:


overview



Maleimides are the preferred functionality for labeling of small molecules such as peptide and oligo-nucleotides. Maleimides form bonds with thiols at relatively mild conditions and with reasonable yields. Maleimides are less susceptible to hydrolysis in comparison with NHS esters.

We would like to refer you to our labeling procedures for specific guidance on how to label with NHS-esters or maleimides.

Click Chemistry Labels:
click

SETA BioMedicals also offers reagents for click chemistry. Several of our most sensitive Seta Dyes are now available as azides to be reacted with C-C-triple bonds in either a Cu(I)-catalized 1,3-dipolar cycloaddition reaction to a triazole (see figure). This reaction proceeds in aqueous environments with adequate efficiency.

Strain-mediated cyclization reactions without the need for a Cu-catalyst using cyclo-octynes were also reported. Initially, the copper(I)-catalyzed azide-alkyne cycloaddition was the only click reaction. The metal-free reactions have a wider applicability and are compatible for cell-based applications.

SETA BioMedicals in collaboration with CCT offers a series of highly fluorescent cyclo-octynes for cell-based applications that allow to perform click chemistry reactions without the use of a Cu(I) catalyst.

Seta Dye Series:


The Seta dye series spans the whole visible range and the NIR. These labels cover most of the important wavelength that are available from common laser sources. Our newly introduced fluorescent labels Seta-550, Seta-650 and Seta-750 are direct replacements of the conventional Cy series Cy3, Cy5 and Cy7 with more or less the same excitation and emission wavelengths as these but with higher quantum yields and photostability. Additional labels in this series will be released soon.


Long-Wavelength SeTau Series (Squaraine Rotaxanes):


The long-wavelength Squaraine-rotaxane series is a new, next generation class of dyes that combines extremely high quantum yields in water (up to 60%) with high extinction coefficients in the order of 150-365,000 M-1. cm-1. These dyes were developed by SETA BioMedicals in order to overcome some of the shortcomings of conventional dyes such as low sensitivity, low stability against oxygen species and low photostability. SeTau dyes are available in reactive, water-soluble forms as carboxy, NHS ester and maleimides but also as hydrophobic probes for imaging.


Short-Wavelength SeTau Series:


SETA BioMedicals also offers long lifetime labels with lifetimes in the range between 32 and 9 ns. These dyes are excitable in the UV or blue region and are useful as polarization probes for the measurement of high MW analytes (small proteins). SeTau-425-NHS for example was used to measure high molecular-weight analytes such as BSA directly in a FP assay. Importantly the FLT of these labels does not change upon labeling or binding to proteins or analytes. These labels exhibit long lifetimes that are not environment sensitive.


Square Series:


The Square series of dyes are more hydrophobic in nature and thus they exhibit a strong environment sensitive behavior and are therefore very suitable to trace binding events between a labeled, low-molecular-weight analyte and a larger biomolecules. The read out parameter can either be intensity or lifetime in this case. More detailed information about specific labels in this series e.g. K8-1407 (pH-sensitive label), K8-1300 or K8-1352 are provided in the Applications section. 


Fluorescent Probes:

SETA BioMedicals offers probes for various types of applications:

Some of these specific probes are targeted to the measurement of changes in lipid bilayer hydration of cells, measurement of cholesterol in biological fluids, or probes for proteins analysis. Square probes are probably one of the most sensitive dyes for the quantitative analysis of proteins.

We also offer a new type of extremely photstable and bright probes for cellular imaging which are based on the long-wavelength SeTau dye series. Our pH-sensitive probes Square-650-pH is the only probe of its kind which shows lifetime sensitive responses in the physiological pH range. It is well suited for measurements of internal conversion and endocytic events.


Phycobiliproteins:


Phycobiliproteins are water-soluble, light-harvesting proteins that are highly fluorescent due to the incorporation of tetrapyrrole chromophores. They are isolated from cyanobacteria and eukaryotic algae. Phycobiliproteins hold the record for the largest number of photons emitted before bleaching – they are one of the most sensitive fluorescent reporters known. SETA BioMedicals offers
lyophilized versions of these proteins at very reasonable prices.



Dyes for Beads:


SETA BioMedicals also offers extremely bright fluorescent classification dyes for encoding of microspheres (beads), used in High-Throughput Screening (HTS) and Multiplexed Analysis Systems.

More information about the applications-oriented use of the dyes is given under Applications.


Non-fluorescent Quenchers:


Non-fluorescent quencher molecules for FRET applications, both reactive and non-reactive versions are also available.
 
 
Product Specifications:
Excitation Light Sources Free Dye Dye Conjugate
Product Number Product Name Target
Group		 380405 436488532594635670SolventλAb max. [nm]ε [M–1
cm–1]		 λEm max. [nm]QY [%] Carrier
PB 7.4		λAb max. [nm]λEm max. [nm]QY [%]
                       
K6-1037 K37 • • • 449 29500 632 0.7 434 540 49
K7-545 SeTau-425-NHS NH2 • 425 4200 545 39 0.0 427 528 16
K7-547 SeTau 405 NHS NH2 • • PB 7.4 405 13800 518 80 BSA, D/P=8 406 518 20
K7-549 SeTau-404-NHS NH2 • • PB 7.4 402 10600 515 52 BSA, D/P=2 402 503
K8-1252 Square-635-di-NHS NH2 • • PB 7.4 636 216000 648 6 BSA, D/P=0.5 652 665 20
K8-1262 Seta-635-di-NHS NH2 • • PB 7.4 640 120000 653 5 IgG, D/P=1 646 658 13
K8-1342 Seta-670-NHS NH2 • • • PB 7.4 667 179000 688 7 BSA, D/P=1 681 695 35
K8-1351 Square-660-COOH • • • • PB 7.4 658 182000 678 3 6g/L BSA 679 696 24
K8-1352 Square-660-NHS NH2 • • PB 7.4 658 182000 677 3 BSA, D/P=1 676 695 13
K8-1355 Square-670H-COOH • • • MeOH 668 208000 690 10 1g/L BSA 688 708 62
K8-1357 Square-685-NHS NH2 • • • • CHCl3 683 208000 703 45
K8-1500 Square-655 • • • MeOH 628 177000 654 0.09 1 g/L BSA 652 672 80
K8-1641 Seta-632-Maleimide SH • PB 7.4 633 270000 642 5
K8-1642 Seta-632-NHS NH2 • PB 7.4 632 280000 641 6 IgG, D/P=1 637 647 18
K8-1649 SQ-740-NHS NH2 • • Water 740 44000
K8-1663 Seta-633-NHS NH2 • PB 7.4 633 250000 644 7 IgG, D/P=1 637 647 26
K8-1669 Seta-635-NH-NHS NH2 • PB 7.4 638 136000 655 31
K8-1672 Seta-646-NHS NH2 • PB 7.4 646 207000 656 10 IgG, D/P=1 650 661 33
K8-1682 Seta-660-di-NHS NH2 • PB 7.4 661 151000 672 11 IgG, D/P=1 665 675 49
K8-1902 SQ-565-NHS NH2 • • Water 560 22000
K8-2602 SQ-755-di-NHS NH2 • Water 756 200000
K8-3002 Seta-470-NHS NH2 • • • PB 7.4 469 50000 521 2 BSA, D/P=1 469 515 28
K8-3335 Seta-555-NHS NH2 • PB 7.4 556 155000 570 12 IgG, D/P=1 654 571 23
K9-4112 SeTau-655-di-NHS NH2 • PB 7.4 665 200000 673 25 IgG, D/P=1 657 675 31
K9-4113 SeTau-665-di-NHS NH2 • • PB 7.4 665 140000 715 62 IgG, D/P=1 664 709 43
K9-4142 SeTau-647-di-NHS NH2 • PB 7.4 650 368000 694 65 IgG, D/P=0.5 649 691 58
K1-204 SeTau-380-NHS NH2 • Water 270 23800 480 56 BSA, 383 468 44
K8-5035 Seta-650-NHS NH2 • PB 7.4 651 172000 671 28 IgG, D/P=1 654 673 37
K8-7035 Seta-750-NHS NH2 • PB 7.4 750 235000 779 22 IgG, D/P=1 755 783 24
K8-1261 Square-650-NHS NH2 • • • CHCl3 649 138000 664 17
K8-1320 Square-670-di-NHS NH2 • • PB 7.4 667 188000 685 7 BSA, D/P=1 682 701 23
K8-1344 Seta-665-NHS NH2 • • • PB 7.4 667 118000 683 3 BSA, D/P=1 682 697 38
K8-1367 Seta-670-NH-di-NHS NH2 • • PB 7.4 672 93000 696 15 IgG, D/P=1 684 703 7
K8-1377 Seta-690-NH-di-NHS NH2 • • PB 7.4 693 113000 714 14 BSA, D/P=1 711 726 14
K8-1382 Seta-660-ammonium-NHS NH2 • • • • • PB 7.4 658 150000 678 2 BSA, D/P=0.3 678 696 10
K8-1446 Seta-700-NHS NH2 • • • • • CHCl3 702 54000 728 22
K8-1752 Square-635-B-di-NHS NH2 • • • PB 7.4 628 103000 649 5 BSA, D/P=1 645 668 27
K8-1667 Seta-635-NH-di-NHS NH2 • PB 7.4 640 188000 656 33 IgG, D/P=1 646 663 21
K8-1350 Square-670-Carboxy • • • PB 7.4 662 207000 680 1.7 6 g/L BSA 658 676 29
K8-1300 Square-670-di-COOH • • • PB 7.4 667 207000 685 7 6g/L BSA 678 698 28
K8-5036 Seta-650-maleimide SH • PB 7.4 652 210000 672 28
K8-7038 Seta-750-Maleimide SH • PB 7.4 752 235000 779 22 IgG, D/P=1 755 783 24
K9-4149 Seta-647-NHS NH2 • PB 7.4 649 200000 695 61 IgG, D/P=1 648 694 59
K9-4119 SeTau-665-NHS NH2 • • • PB 7.4 664 160000 712 53 IgG, D/P=1.2 653 716 50
K8-1922 SQ-780-NHS NH2 • • MeOH 782 115000
K8-1407 Square-650-pH-NHS • PB 7.4 653 135000 671 16 IgG, D/P=1 662 677 7
 
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