|
Intensity Probes
Measurement of changes in lipid bilayer hydration in cells: Square 670 (K8-1350) is as an effective red emitting fluorescent probe for examining membrane-related processes, e.g. drug-influenced changes in bilayer hydration [19].
|
| Product | Pack Size | Product Code |
| Square-670 |
1 mg
|
K8-1350-1mg |
Pricing and Availability
Ordering
|
| Measurement of cholesterol: Square 440 is as an effective and specific fluorescent probe for the measurement of cholesterol in biological fluids. The probe has an absorption maximum of 440 nm, an extinction coefficient of about 30,000 M-1.cm-1 and a large Stokes’ shift of over 100 nm. The compound is also capable of measuring total cholesterol and triglycerides in serum. Here the measurement range of the probe is |
 |
0 – 6 mg/ml. It is a mix and read, non-enzymatic measurement and therefore easy to automate. Detailed measurement protocols are available. Please contact us for more information.
|
| Product | Pack Size | Product Code |
|
|
1 mg
|
K6-1010 |
Pricing and Availability
Ordering
|
|
|
Fluorescence intensity of Square 440 vs. cholesterol concentration
|
|
|
|
| |
|
Protein Analysis: Dyes such as Square-655 (K8-1500) and Square-670H (K8-1355) exhibit a substantial fluorescence intensity increase (up to 190 times) in the presence of BSA. Fluorescence enhancement of the dyes in the presence of other albumins (HSA and ovalbumin) is lower (40 times). The large fluorescence enhancement in presence of proteins makes them valuable dyes for the visualization and quantification of proteins in gel-electrophoresis applications.
|
 |
| |
Fluorescence intensity of Square 665 vs.
BSA concentration
|
|
| Square dyes are the most sensitive dyes on the market for the detection of proteins. The protein-bound forms of Square dyes exhibit absorption and emission maxima between 640 and 750 nm, very high extinction coefficients between 104,000–208,000 M–1cm–1 and quantum yields as high as 80%. |
| Product | Pack Size | Product Code |
|
|
1 mg
|
K8-1500-1mg |
|
|
1 mg |
K8-1355-1mg |
Pricing and Availability
Ordering |
| |
|
|
| |
| Squaraine-Rotaxane imaging probes for live and fixed cells: Fluorescent probes such as SeTau 650 (K9-4116) exhibit extremely high chemical and photostability and can therefore well suited for imaging applications. |
|
|
| These probes are ethyl ester derivative and therefore passively penetrate the cell membranes. Once inside they are hydrolyzed by esterases thereby forming carboxyl groups that are cell-impermeable. These probes can be structurally modified for targeting different cellular locations for in vitro and in vivo optical imaging of live and fixed cells. Images stained with these probes are stable for hundreds of hours. Please contact us at 217 417 2160 for more information. |
| |
| Product | Pack Size | Product Code |
|
|
1 mg
|
K9-4116-1mg |
|
|
1 mg |
K9-4145-1mg |
|
|
1 mg |
K9-1604-1mg |
Pricing and Availability
Ordering
|
|
|
|
| |
| pH-measurement in cells: Probes such as K8-1405 (carboxyl version of K8-1407) and K8-1407 exhibit pKa’s in the physiologically pH range and are very useful to determine the intercellular pH in cells. For more information about these dyes we refer you to the specs sheet of K8-1407 and the published literature. |
| |
|
|
| Product | Pack Size | Product Code |
|
|
1 mg
|
K8-1407-1mg |
|
|
1 mg |
K8-1405-1mg |
Pricing and Availability
Ordering
|
|
|
Live and Dead Cells Staining Kits: Our live and dead cell staining kits help to distinguish between live and dead cells by a very simple principle: live cells react with the fluorescent dye only on their surface and yield weakly fluorescent cells. Cells with disrupted cell membranes yield cells which are brightly fluorescent (see image below). The intensities of necrotic and live cells are very different which allows easy discrimination between the 2 cell populations. Staining can be done in covalent as well as non-covalent fashion. Covalent staining is necessary if fixation of the cells by formaldehyde is required. The assays can also be used to detect necrotic cells by microscopy.
|
|
|
SETA BioMedicals offers a covalent and a non-covalent staining kit for this type of measurements. The yellowish green color of these dyes is excitable with the 470 or 488 nm lasers. Each kit contains enough dye to perform 200 - 250 flow cytometric assays.
|
|
|
|
|
| |
Dinitro-reductase enzyme activity in cells: Our novel dye-substrates containing dinitro-benzyl-substituents show favorable properties for use of imaging of dinitro-reductase enzyme activity in cells.
|
| |
 |
| |
Comparison of the signals of a
fluorogenic enzyme-substrate,
before (right) and after trans-
formation (left) by the DNT enzyme.
|
|
Visualization of nitro-reductase (DNT) enzyme activity in cells can be used as a non-invasive, live-cell reporter system. A cell-permeable fluorogenic substrate is used to report on the enzyme activity, which is based on the increase of the fluorescence signals upon reduction of the nitro-group on the enzyme substrate (Figure). This non-destructive method offers the ability to screen out non-responding cells, analysis of individual cell responses, and measurement of a second fluorescent event occurring within the same cell.
|
| |
| Product | Pack Size | Product Code |
|
DNB-Square 670
|
1 mg
|
K8-1359-1mg |
Pricing and Availability
Ordering
|
|
|
| |
|
| |
|
Probes for the evaluation of cryoprotection: Cryoprotectors (CPs) are important reagents that help to prevent biological matter such as blood, tissues and cells from degradation during low-temperature storage. Fluorescent dyes are useful tools to investigate the interaction of CPs with biological species. Long-term and low temperature storage of cells is an important process for biomedical research and transplantation medicine. Biomembranes are cellular structures that are strongly affected by the freeze-thawing process.
The freeze-thawing experiments with yeast cells using cryoprotectors have demonstrated that 2-DAB and 3-DAB are fluorescent probes that enable not only to distinguish damaged from undamaged cells, but also allow quantitative estimation of the extent of damage by the cryogenic treatment. These fluorescent probes are therefore very useful to quickly assess the effects of cryopreservation on cells using fluorescence imaging.
|
| |
 |
 |
|
Saccharomyces Cerevisiae cells stained
with 3-DAB before undergoing the cycle
cryoprotection.
|
Saccharomyces Cerevisiae cells stained
with dye 3-DAB after freeze-thawing undergoing
the cycle cryoprotection. Partially damaged
cells exhibit brighter fluorescence as
compared to living cells.
|
|
| Product | Pack Size | Product Code |
|
|
1 mg
|
K6-207-1mg |
|
|
1 mg |
K6-208-1mg |
Pricing and Availability
Ordering
|
