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t1  Fluorescence Polarization Standards t1
 
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Fluorescence Polarization Standards

The table below contains information about fluorescence polarization standards that can be used for calibration. They exhibit high polarization values at room temperature in water. Some of these standards are abailable from SETA BioMedicals.

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Polarization Standard Polarization [mP] Solvent 25°C Excitation Range [nm] Emission [nm]
Rose Bengal
344 Water 510-550 560-590
Indocyanine Green
379 Water 700-800 822
Square-660-COOH (K8-1351)
333 Water 600-670 680-700
Square-470-COOH (K8-3001)
310 Water 440-490 515-530
 
 
 
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