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Lifetime Probes
Probes such as Square-670-di-COOH (K8-1300) show large changes in FLT upon binding to either proteins or cell-surfaces. K8-1300 is water-soluble but with a strong affinity for hydrophobic regions of biomolecules.
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Comparison of the frequency responses of
K8-1300 before and after binding to protein.
(τfree = 460 ps;τbound = 3.26 ns)
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Comparison of the intensity decays of
K8-1300 before and after binding to protein.
(τfree= 460 ps; τbound = 3.26 ns)
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Pricing and Availability
Ordering
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| K8-1407 is a NIR probe that exhibits a pKa around 7 and is therefore useful for measurement of pH changes in the physiologically relevant pH range from pH 8 to pH 6.5. Importantly both forms (protoneated as well as unprotonated) are fluorescent and they exhibit different lifetimes which makes this compound also useful for lifetime-based applications (see Figure below). For more information about these dyes we refer you to the specs sheet of K8-1407 and the published literature. |
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